ACTA Pharmaceutica Sciencia 2009 , Vol 51 , Num 3
EFFECT OF SILYMARIN AND POMEGRANATE PRETREATMENT ON INTESTINAL TRANSPORT OF BUSPIRONE ACROSS RAT INTESTINE
To investigate the effect of silymarin and pomegranate juice pretreatment on the transport of buspirone, a CYP3A4 substrate across the rat intestine The transport of buspirone across rat intestine (duodenum, jejunum and ileum) was studied by using non-everted sac method. The rats were pretreated with silymarin and pomegranate juice for 7 days. The rats were sacrificed by using anesthetic ether. The intestinal segments were isolated and used for the studies. The probe drug (buspirone) solution was placed in the isolated intestinal sac. Samples were collected at preset time points and replaced with fresh buffer. The drug content in the samples was estimated using high performance liquid chromatography method. Control experiments were also performed. The results reveal that there was a significant (p<0.05) difference compared to control, in the transport of buspirone from the intestinal sacs which were pretreated with silymarin and pomegranate juice. It suggests that both silymarin and pomegranate juice might be acting by inhibiting the transporters and enzymes which are responsible for transport/metabolism of buspirone. From the results it can be concluded that silymarin and pomegranate juice might be acting by inhibiting CYP3A4 enzymes as buspirone is extensively metabolized by CYP3A4. Further studies are recommended to prove their effects in human beings. Keywords :

Istanbul Medipol University