Journal of Critical and Intensive Care Online Early
Matrix Metalloproteinase-3 E45K Polymorphism is Associated with the Risk of Sepsis in Turkish Population: A Preliminary Study
Merve Nur ATAS 1 ,Leyla ACAR 1 ,Nihan YAPICI 2 ,Nazan Atalan OZLEN 3 ,Arzu ERGEN 1
1Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkiye
2Department of Anesthesiology, Dr. Siyami Ersek Thoracic and Cardiovascular Surgery Training and Research Hospital, University of Health Sciences, Istanbul, Turkiye
3Department of Anesthesiology, Faculty of Medicine, Marmara University, Istanbul, Turkiye
DOI : 10.37678/dcybd.2024.3499 Aim: Sepsis is a disease in which an abnormal immune response triggered by an infection causes mortality. Matrix metalloproteinases (MMPs), in addition to their proteolytic activity in physiological conditions, contribute to inflammation resulting from this abnormal immune response in sepsis. The aim of the study was to investigate the effect of MMP-3 gene variation on the risk of sepsis development in the Turkish population.

Study Design: A case-control study

Material and Methods: In this study, E45K and T102T polymorphisms of MMP-3 were examined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods in 78 sepsis patients and 81 healthy controls in Turkish population.

Results: According to the results, frequency of carrying E45K G allele (p=0.004) significantly increased in sepsis patients compared to healthy controls. In addition, healthy controls had increased E45K AA genotype (p=0.004). On the other hand, we found that having the E45K GG genotype (p<0.001) increased the risk of developing sepsis in patients with Chronic Obstructive Pulmonary Disease (COPD). Accordingly, irrespective of patients' presence or absence COPD, carrying the G allele, whether homozygous or heterozygous, appears to be a risk factor for sepsis. However, no significant changes in allele frequencies or genotypes were observed for the T102T polymorphism in both groups.

Conclusion: In conclusion, our primary findings showed that carrying the E45K G allele may be associated with sepsis. Keywords : Sepsis, Polymorphism, Matrix metalloproteinase-3, COPD